Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14185 and 14186 are separated by 636 nucleotides14186 and 14187 are separated by 194 nucleotides14187 and 14188 are separated by 184 nucleotides14188 and 14189 are separated by 102 nucleotides mRNA_5817: 14185 - K09680 coaW type II pantothenate kinase, at 1,013,105 to 1,015,263 14185 mRNA_5818: 14186 - K14857 SPB1, FTSJ3 AdoMet-dependent rRNA methyltransferase SPB1, at 1,015,900 to 1,019,364 14186 mRNA_5819: 14187 - K10761 THG1 tRNA(His) guanylyltransferase, at 1,019,559 to 1,020,828 14187 mRNA_5820: 14188 - K11763 RSC9 chromatin structure-remodeling complex subunit RSC9, at 1,021,013 to 1,023,514 14188 mRNA_5821: 14189 - SUPERFAMILY--SSF52047, at 1,023,617 to 1,025,042 14189
Group Condition mRNA_5817 mRNA_5818 mRNA_5819 mRNA_5820 mRNA_5821
14185 14186 14187 14188 14189
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -1.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.7 -0.3
auxotrophy Dropout Complete N.D. N.D. N.D. -0.9 -0.1
growth on ypd YPD N.D. N.D. N.D. -0.1 -0.8
auxotrophy YNB Glucose N.D. N.D. N.D. -0.5 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.5 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.7 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.2 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.2 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.5 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.4 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.7 +0.3
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