Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14196 and 14197 are separated by 179 nucleotides14197 and 14198 are separated by 452 nucleotides14198 and 14199 are separated by 136 nucleotides14199 and 14200 are separated by 272 nucleotides mRNA_5828: 14196 - KOG0725 Reductases with broad range of substrate specificities, at 1,036,637 to 1,037,941 14196 mRNA_5829: 14197 - K00228 CPOX, hemF coproporphyrinogen III oxidase, at 1,038,121 to 1,039,722 14197 mRNA_5830: 14198 - HMMPfam-Mitochondrial protein Pet127-PF08634, at 1,040,175 to 1,043,789 14198 mRNA_5831: 14199 - KOG1198 Zinc-binding oxidoreductase, at 1,043,926 to 1,045,308 14199 mRNA_5832: 14200 - K02863 RP-L1, MRPL1, rplA large subunit ribosomal protein L1, at 1,045,581 to 1,047,659 14200
Group Condition mRNA_5828 mRNA_5829 mRNA_5830 mRNA_5831 mRNA_5832
14196 14197 14198 14199 14200
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.4 N.D. -0.0 -0.4
growth on ypd YPD -0.1 -0.0 N.D. +0.4 -0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 N.D. -0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 N.D. +0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.2 N.D. +0.3 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 N.D. +0.2 -0.1
auxotrophy Dropout Complete -0.2 +0.3 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 N.D. +0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.2 N.D. +0.6 -0.1
growth on oleic acid YNB Glucose +0.1 +0.2 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.0 N.D. +0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.4 N.D. -0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.2 -0.0 N.D. +0.2 +0.3
auxotrophy YNB Glucose -0.0 +0.4 N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.2 N.D. +0.4 +0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.0 N.D. +0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.4 N.D. +0.3 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.0 N.D. +0.5 +0.5
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