Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14217 and 14218 are separated by 665 nucleotides14218 and 14219 are separated by 543 nucleotides14219 and 14220 are separated by 203 nucleotides14220 and 14221 are separated by 125 nucleotides mRNA_5849: 14217 - SMART-Furin-like repeats-SM00261,SUPERFAMILY--SSF57184, at 1,079,850 to 1,082,159 14217 mRNA_5850: 14218 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF03171,HMMPfam-non-haem dioxygenase in morphine synthesis N-terminal-PF14226,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SUPERFAMILY--SSF51197, at 1,082,825 to 1,084,341 14218 mRNA_5851: 14219 - SUPERFAMILY--SSF51735, at 1,084,885 to 1,085,792 14219 mRNA_5852: 14220 - K14856 SDA1, SDAD1 protein SDA1, at 1,085,996 to 1,089,150 14220 mRNA_5853: 14221 - K15378 SLC45A1_2_4 solute carrier family 45, member 1/2/4, at 1,089,276 to 1,092,744 14221
Group Condition mRNA_5849 mRNA_5850 mRNA_5851 mRNA_5852 mRNA_5853
14217 14218 14219 14220 14221
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.2 +0.4 N.D. -1.4
growth on ypd YPD -0.3 -0.2 -0.6 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 -0.0 +0.2 N.D. -0.6
growth on oleic acid YNB Glucose -0.0 +0.1 -0.5 N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.3 -0.1 +0.1 N.D. -0.4
auxotrophy YNB Glucose -0.3 +0.2 -0.5 N.D. +0.2
auxotrophy Dropout Complete -0.2 +0.0 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.3 +0.4 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.3 +0.6 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 -0.2 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.0 +0.4 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 -0.2 N.D. +0.2
growth on oleic acid YNB Oleic Acid -0.1 +0.1 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.2 +0.6 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.2 +0.5 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.2 +0.5 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.0 -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 +0.6 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 -0.1 N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.3 +0.6 N.D. +0.1
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