Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14218 and 14219 are separated by 543 nucleotides14219 and 14220 are separated by 203 nucleotides14220 and 14221 are separated by 125 nucleotides14221 and 14222 are separated by 296 nucleotides mRNA_5850: 14218 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF03171,HMMPfam-non-haem dioxygenase in morphine synthesis N-terminal-PF14226,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SUPERFAMILY--SSF51197, at 1,082,825 to 1,084,341 14218 mRNA_5851: 14219 - SUPERFAMILY--SSF51735, at 1,084,885 to 1,085,792 14219 mRNA_5852: 14220 - K14856 SDA1, SDAD1 protein SDA1, at 1,085,996 to 1,089,150 14220 mRNA_5853: 14221 - K15378 SLC45A1_2_4 solute carrier family 45, member 1/2/4, at 1,089,276 to 1,092,744 14221 mRNA_5854: 14222 - K12826 SF3A2, SAP62 splicing factor 3A subunit 2, at 1,093,041 to 1,094,048 14222
Group Condition mRNA_5850 mRNA_5851 mRNA_5852 mRNA_5853 mRNA_5854
14218 14219 14220 14221 14222
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.4 N.D. -1.4 N.D.
growth on ypd YPD -0.2 -0.6 N.D. +0.0 N.D.
growth on oleic acid YNB Glucose +0.1 -0.5 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.2 N.D. -0.6 N.D.
growth on ricinoleic acid YNB Glucose -0.1 +0.1 N.D. -0.4 N.D.
auxotrophy Dropout Complete +0.0 -0.2 N.D. +0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.6 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 N.D. +0.1 N.D.
auxotrophy YNB Glucose +0.2 -0.5 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.2 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 N.D. +0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.0 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.4 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.6 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.4 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.5 N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.6 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.5 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.6 N.D. +0.1 N.D.
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