Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14220 and 14221 are separated by 125 nucleotides14221 and 14222 are separated by 296 nucleotides14222 and 14223 are separated by 245 nucleotides14223 and 14224 are separated by 506 nucleotides mRNA_5852: 14220 - K14856 SDA1, SDAD1 protein SDA1, at 1,085,996 to 1,089,150 14220 mRNA_5853: 14221 - K15378 SLC45A1_2_4 solute carrier family 45, member 1/2/4, at 1,089,276 to 1,092,744 14221 mRNA_5854: 14222 - K12826 SF3A2, SAP62 splicing factor 3A subunit 2, at 1,093,041 to 1,094,048 14222 mRNA_5855: 14223 - Hypothetical Protein, at 1,094,294 to 1,098,449 14223 mRNA_5856: 14224 - K03357 APC10, DOC1 anaphase-promoting complex subunit 10, at 1,098,956 to 1,099,820 14224
Group Condition mRNA_5852 mRNA_5853 mRNA_5854 mRNA_5855 mRNA_5856
14220 14221 14222 14223 14224
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.4 N.D. +0.2 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.4 N.D. -0.5 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 N.D. -0.1 -1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. -0.3 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. -0.4 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 N.D. -0.1 -0.5
growth on oleic acid YNB Glucose N.D. -0.2 N.D. -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.9 +0.0
auxotrophy Dropout Complete N.D. +0.0 N.D. +0.3 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. +0.3 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. +0.2 -0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.4 N.D. +0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.6 N.D. +0.3 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. -0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.3 -0.8
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 N.D. -0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 N.D. +0.8 -1.0
growth on ypd YPD N.D. +0.0 N.D. +0.5 +0.3
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