Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14221 and 14222 are separated by 296 nucleotides14222 and 14223 are separated by 245 nucleotides14223 and 14224 are separated by 506 nucleotides14224 and 14225 are separated by 117 nucleotides mRNA_5853: 14221 - K15378 SLC45A1_2_4 solute carrier family 45, member 1/2/4, at 1,089,276 to 1,092,744 14221 mRNA_5854: 14222 - K12826 SF3A2, SAP62 splicing factor 3A subunit 2, at 1,093,041 to 1,094,048 14222 mRNA_5855: 14223 - Hypothetical Protein, at 1,094,294 to 1,098,449 14223 mRNA_5856: 14224 - K03357 APC10, DOC1 anaphase-promoting complex subunit 10, at 1,098,956 to 1,099,820 14224 mRNA_5857: 14225 - K01875 SARS, serS seryl-tRNA synthetase, at 1,099,938 to 1,101,615 14225
Group Condition mRNA_5853 mRNA_5854 mRNA_5855 mRNA_5856 mRNA_5857
14221 14222 14223 14224 14225
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 N.D. +0.2 -0.9 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.5 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.1 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.3 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. -0.4 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.1 -0.5 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. -0.2 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.9 +0.0 N.D.
auxotrophy Dropout Complete +0.0 N.D. +0.3 -1.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.3 -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.2 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. +0.2 -0.8 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.3 -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.4 N.D. +0.3 -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. +0.3 -0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.2 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.2 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.3 -0.8 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.2 -0.2 N.D.
auxotrophy YNB Glucose +0.2 N.D. +0.8 -1.0 N.D.
growth on ypd YPD +0.0 N.D. +0.5 +0.3 N.D.
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