Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14225 and 14226 are separated by 652 nucleotides14226 and 14227 are separated by 424 nucleotides14227 and 14228 are separated by 213 nucleotides14228 and 14229 are separated by 333 nucleotides mRNA_5857: 14225 - K01875 SARS, serS seryl-tRNA synthetase, at 1,099,938 to 1,101,615 14225 mRNA_5858: 14226 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SUPERFAMILY--SSF57959, at 1,102,268 to 1,103,746 14226 mRNA_5859: 14227 - BLAST probable atp-binding cassette transporter [Ceraceosor..., at 1,104,171 to 1,104,734 14227 mRNA_5860: 14228 - K19770 RNASEK ribonuclease kappa, at 1,104,948 to 1,105,402 14228 mRNA_5861: 14229 - K16261 YAT yeast amino acid transporter, at 1,105,736 to 1,108,814 14229
Group Condition mRNA_5857 mRNA_5858 mRNA_5859 mRNA_5860 mRNA_5861
14225 14226 14227 14228 14229
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. -0.7 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 N.D. -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. -0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.6 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. -0.6 +0.2
growth on oleic acid YNB Glucose N.D. -0.0 N.D. +0.0 -0.2
auxotrophy Dropout Complete N.D. -0.0 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. -0.1 +0.1
auxotrophy YNB Glucose N.D. +0.0 N.D. +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 N.D. -0.2 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 N.D. +0.6 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. +0.1 +0.2
growth on ypd YPD N.D. +0.0 N.D. +0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.6 +0.1
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