Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14231 and 14232 are separated by 210 nucleotides14232 and 14233 are separated by 275 nucleotides14233 and 14234 are separated by 284 nucleotides14234 and 14235 are separated by 222 nucleotides mRNA_5863: 14231 - K09281 K09281 copper-fist transcription factor, at 1,112,014 to 1,113,676 14231 mRNA_5864: 14232 - K01322 PREP prolyl oligopeptidase, at 1,113,887 to 1,116,821 14232 mRNA_5865: 14233 - KOG0800 FOG Predicted E3 ubiquitin ligase, at 1,117,097 to 1,118,879 14233 mRNA_5866: 14234 - KOG1454 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily), at 1,119,164 to 1,122,161 14234 mRNA_5867: 14235 - K02950 RP-S12, MRPS12, rpsL small subunit ribosomal protein S12, at 1,122,384 to 1,123,037 14235
Group Condition mRNA_5863 mRNA_5864 mRNA_5865 mRNA_5866 mRNA_5867
14231 14232 14233 14234 14235
ypd buoyancy 40 hrs No Buoyancy Separation -2.7 -0.1 +0.0 -0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.2 -0.1 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -1.5 -0.0 -0.2 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.1 -0.1 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.3 -0.2 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 -0.1 +0.2 -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.5 -0.0 +0.3 -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.1 -0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.2 -0.3 -0.3 N.D.
growth on oleic acid YNB Glucose +0.2 -0.3 +0.0 -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.5 -0.1 -0.3 -0.3 N.D.
auxotrophy Dropout Complete +0.9 -0.1 -0.2 -0.7 N.D.
auxotrophy YNB Glucose +0.8 -0.0 -0.1 -0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.1 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.0 -0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +1.2 +0.1 -0.1 -0.5 N.D.
growth on ypd YPD +1.7 +0.0 +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.9 -0.0 +0.1 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +3.2 -0.0 +0.4 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +3.5 -0.2 +0.5 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +3.4 +0.0 +0.4 +0.7 N.D.
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