Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14232 and 14233 are separated by 275 nucleotides14233 and 14234 are separated by 284 nucleotides14234 and 14235 are separated by 222 nucleotides14235 and 14236 are separated by 203 nucleotides mRNA_5864: 14232 - K01322 PREP prolyl oligopeptidase, at 1,113,887 to 1,116,821 14232 mRNA_5865: 14233 - KOG0800 FOG Predicted E3 ubiquitin ligase, at 1,117,097 to 1,118,879 14233 mRNA_5866: 14234 - KOG1454 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily), at 1,119,164 to 1,122,161 14234 mRNA_5867: 14235 - K02950 RP-S12, MRPS12, rpsL small subunit ribosomal protein S12, at 1,122,384 to 1,123,037 14235 mRNA_5868: 14236 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF03171,HMMPfam-non-haem dioxygenase in morphine synthesis N-terminal-PF14226,PRINTS-Isopenicillin N synthase signature-PR00682,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SUPERFAMILY--SSF51197, at 1,123,241 to 1,124,381 14236
Group Condition mRNA_5864 mRNA_5865 mRNA_5866 mRNA_5867 mRNA_5868
14232 14233 14234 14235 14236
growth on oleic acid YNB Glucose -0.3 +0.0 -0.3 N.D. -0.6
auxotrophy Dropout Complete -0.1 -0.2 -0.7 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.3 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.3 -0.3 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.2 -0.2 N.D. -0.2
auxotrophy YNB Glucose -0.0 -0.1 -0.5 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.0 -0.4 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 -0.5 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.2 +0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.0 +0.3 -0.4 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 +0.0 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.1 -0.3 N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.2 +0.1 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 -0.4 N.D. +0.7
growth on ypd YPD +0.0 +0.1 -0.2 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.0 +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.1 +0.6 N.D. +0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.5 +0.6 N.D. +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.4 +0.7 N.D. +1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.4 +0.7 N.D. +1.2
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