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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14233 and 14234 are separated by 284 nucleotides
14234 and 14235 are separated by 222 nucleotides
14235 and 14236 are separated by 203 nucleotides
14236 and 14237 are separated by 341 nucleotides
mRNA_5865: 14233 - KOG0800 FOG Predicted E3 ubiquitin ligase, at 1,117,097 to 1,118,879
14233
mRNA_5866: 14234 - KOG1454 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily), at 1,119,164 to 1,122,161
14234
mRNA_5867: 14235 - K02950 RP-S12, MRPS12, rpsL small subunit ribosomal protein S12, at 1,122,384 to 1,123,037
14235
mRNA_5868: 14236 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF03171,HMMPfam-non-haem dioxygenase in morphine synthesis N-terminal-PF14226,PRINTS-Isopenicillin N synthase signature-PR00682,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SUPERFAMILY--SSF51197, at 1,123,241 to 1,124,381
14236
mRNA_5869: 14237 - K10808 RRM2 ribonucleoside-diphosphate reductase subunit M2, at 1,124,723 to 1,126,548
14237
Group
Condition
mRNA
_5865
mRNA
_5866
mRNA
_5867
mRNA
_5868
mRNA
_5869
14233
14234
14235
14236
14237
auxotrophy
Dropout Complete
-0.2
-0.7
N.D.
-0.2
N.D.
growth on oleic acid
YNB Glucose
+0.0
-0.3
N.D.
-0.6
N.D.
growth on oleic acid
YNB Oleic Acid
-0.3
-0.3
N.D.
-0.3
N.D.
auxotrophy
YNB Glucose
-0.1
-0.5
N.D.
-0.1
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.3
-0.3
N.D.
-0.1
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.1
-0.5
N.D.
+0.1
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.2
-0.2
N.D.
-0.2
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
+0.0
-0.4
N.D.
-0.0
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.2
+0.1
N.D.
-0.3
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.0
N.D.
-0.0
N.D.
growth on ricinoleic acid
YNB Glucose
+0.3
-0.4
N.D.
+0.1
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.1
N.D.
+0.2
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
-0.3
N.D.
+0.5
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.2
-0.1
N.D.
+0.0
N.D.
growth on ypd
YPD
+0.1
-0.2
N.D.
+0.3
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
-0.4
N.D.
+0.7
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.0
+0.1
N.D.
+0.4
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
+0.6
N.D.
+0.9
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.5
+0.6
N.D.
+0.9
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.4
+0.7
N.D.
+1.1
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.4
+0.7
N.D.
+1.2
N.D.
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