Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14241 and 14242 are separated by 834 nucleotides14242 and 14243 are separated by 202 nucleotides14243 and 14244 are separated by 1042 nucleotides14244 and 14245 are separated by 345 nucleotides mRNA_5873: 14241 - K00761 upp, UPRT uracil phosphoribosyltransferase, at 1,136,080 to 1,137,305 14241 mRNA_5874: 14242 - K04536 GNB1 guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, at 1,138,140 to 1,139,450 14242 mRNA_5875: 14243 - Hypothetical Protein, at 1,139,653 to 1,140,264 14243 mRNA_5876: 14244 - K14537 NUG2, GNL2 nuclear GTP-binding protein, at 1,141,307 to 1,144,033 14244 mRNA_5877: 14245 - K00624 E2.3.1.7 carnitine O-acetyltransferase, at 1,144,379 to 1,146,733 14245
Group Condition mRNA_5873 mRNA_5874 mRNA_5875 mRNA_5876 mRNA_5877
14241 14242 14243 14244 14245
facs on lipid accumulation Sorted Low BODIPY Gate -4.6 +0.4 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -3.1 -0.0 -0.2 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -1.3 -0.4 +0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +1.0 +0.2 N.D. -3.0
growth on oleic acid YNB Oleic Acid +0.2 +0.5 +0.2 N.D. -2.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +1.3 +0.4 N.D. -2.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.4 -0.0 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.1 -0.0 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.2 +0.6 +0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.2 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 -0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.5 -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.3 -0.0 N.D. -0.2
growth on ypd YPD +0.1 +0.1 -0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.4 +0.0 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 +0.1 N.D. -0.3
auxotrophy Dropout Complete +0.1 +0.3 -0.0 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.7 +0.1 N.D. -0.2
auxotrophy YNB Glucose +0.4 +0.5 +0.0 N.D. -0.2
growth on oleic acid YNB Glucose +0.2 +0.4 +0.2 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.5 +0.7 +0.0 N.D. +0.1
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