Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14247 and 14248 are separated by 275 nucleotides14248 and 14249 are separated by 516 nucleotides14249 and 14250 are separated by 305 nucleotides14250 and 14251 are separated by 86 nucleotides mRNA_5879: 14247 - KOG2624 Triglyceride lipase-cholesterol esterase, at 1,149,162 to 1,152,280 14247 mRNA_5880: 14248 - HMMPfam-Transglycosylase SLT domain-PF01464,SUPERFAMILY--SSF53955, at 1,152,556 to 1,154,002 14248 mRNA_5881: 14249 - BLAST related to embryonic protein DC-8 [Ustilago hordei], at 1,154,519 to 1,154,935 14249 mRNA_5882: 14250 - K15732 CTDP1, FCP1 RNA polymerase II subunit A C-terminal domain phosphatase, at 1,155,241 to 1,158,435 14250 mRNA_5883: 14251 - K09668 LARGE glycosyltransferase-like protein LARGE, at 1,158,522 to 1,160,297 14251
Group Condition mRNA_5879 mRNA_5880 mRNA_5881 mRNA_5882 mRNA_5883
14247 14248 14249 14250 14251
growth on ypd YPD -0.3 -0.1 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. N.D. -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.0 N.D. N.D. -0.0
auxotrophy Dropout Complete -0.0 -0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.3 N.D. N.D. -0.7
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 N.D. N.D. +0.1
auxotrophy YNB Glucose -0.2 +0.0 N.D. N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.0 +0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.3 N.D. N.D. -0.2
growth on oleic acid YNB Glucose +0.1 +0.1 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.0 N.D. N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.1 N.D. N.D. +0.6
growth on oleic acid YNB Oleic Acid -0.1 +0.2 N.D. N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 N.D. N.D. +0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.4 N.D. N.D. +0.7
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