Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14249 and 14250 are separated by 305 nucleotides14250 and 14251 are separated by 86 nucleotides14251 and 14252 are separated by 251 nucleotides14252 and 14253 are separated by 168 nucleotides mRNA_5881: 14249 - BLAST related to embryonic protein DC-8 [Ustilago hordei], at 1,154,519 to 1,154,935 14249 mRNA_5882: 14250 - K15732 CTDP1, FCP1 RNA polymerase II subunit A C-terminal domain phosphatase, at 1,155,241 to 1,158,435 14250 mRNA_5883: 14251 - K09668 LARGE glycosyltransferase-like protein LARGE, at 1,158,522 to 1,160,297 14251 mRNA_5884: 14252 - K13830 ARO1 pentafunctional AROM polypeptide, at 1,160,549 to 1,165,878 14252 mRNA_5885: 14253 - KOG1424 Predicted GTP-binding protein MMR1, at 1,166,047 to 1,168,638 14253
Group Condition mRNA_5881 mRNA_5882 mRNA_5883 mRNA_5884 mRNA_5885
14249 14250 14251 14252 14253
growth on ypd YPD N.D. N.D. -0.3 N.D. -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.5 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 N.D. -0.7
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.5 N.D. -0.7
auxotrophy Dropout Complete N.D. N.D. -0.1 N.D. -0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 N.D. +0.1
auxotrophy YNB Glucose N.D. N.D. +0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.7 N.D. +0.9
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.4 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.6 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.6 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.7 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.5 N.D. +0.5
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