Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14256 and 14257 are separated by 895 nucleotides14257 and 14258 are separated by 344 nucleotides14258 and 14259 are separated by 2247 nucleotides14259 and 14260 are separated by 177 nucleotides mRNA_5888: 14256 - K00688 PYG, glgP glycogen phosphorylase, at 1,171,973 to 1,175,579 14256 mRNA_5889: 14257 - K02977 RP-S27Ae, RPS27A small subunit ribosomal protein S27Ae, at 1,176,475 to 1,177,214 14257 mRNA_5890: 14258 - K01876 DARS, aspS aspartyl-tRNA synthetase, at 1,177,559 to 1,180,094 14258 mRNA_5891: 14259 - K11788 ADE5 phosphoribosylamine--glycine ligase / phosphoribosylformylglycinamidine cyclo-ligase, at 1,182,342 to 1,185,171 14259 mRNA_5892: 14260 - Hypothetical Protein, at 1,185,349 to 1,185,740 14260
Group Condition mRNA_5888 mRNA_5889 mRNA_5890 mRNA_5891 mRNA_5892
14256 14257 14258 14259 14260
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.1 N.D. -4.7 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.8 N.D. -5.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -1.6 N.D. -3.6 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.8 N.D. -4.5 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -2.1 N.D. -3.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 -0.8 N.D. -4.9 -0.1
auxotrophy YNB Glucose +0.0 -0.8 N.D. -4.4 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -1.7 N.D. -3.9 +0.7
growth on oleic acid YNB Glucose -0.2 -0.3 N.D. -4.4 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.0 -1.1 N.D. -2.9 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.7 N.D. -3.5 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.3 N.D. -2.9 -0.5
growth on ricinoleic acid YNB Glucose +0.0 +0.3 N.D. -3.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -1.0 N.D. -1.8 -0.3
auxotrophy Dropout Complete -0.1 -2.6 N.D. +0.5 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.3 N.D. -2.0 -0.1
growth on oleic acid YNB Oleic Acid +0.1 -0.3 N.D. -2.4 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 N.D. -1.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.0 N.D. -0.7 -0.1
growth on ypd YPD -0.1 +1.0 N.D. -1.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -1.7 N.D. +2.3 +0.1
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