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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14257 and 14258 are separated by 344 nucleotides
14258 and 14259 are separated by 2247 nucleotides
14259 and 14260 are separated by 177 nucleotides
14260 and 14261 are separated by 140 nucleotides
mRNA_5889: 14257 - K02977 RP-S27Ae, RPS27A small subunit ribosomal protein S27Ae, at 1,176,475 to 1,177,214
14257
mRNA_5890: 14258 - K01876 DARS, aspS aspartyl-tRNA synthetase, at 1,177,559 to 1,180,094
14258
mRNA_5891: 14259 - K11788 ADE5 phosphoribosylamine--glycine ligase / phosphoribosylformylglycinamidine cyclo-ligase, at 1,182,342 to 1,185,171
14259
mRNA_5892: 14260 - Hypothetical Protein, at 1,185,349 to 1,185,740
14260
mRNA_5893: 14261 - KOG1748 Acyl carrier protein/NADH-ubiquinone oxidoreductase, NDUFAB1/SDAP subunit, at 1,185,881 to 1,186,514
14261
Group
Condition
mRNA
_5889
mRNA
_5890
mRNA
_5891
mRNA
_5892
mRNA
_5893
14257
14258
14259
14260
14261
facs on lipid accumulation
Sorted No BODIPY Gate
-1.1
N.D.
-4.7
-0.4
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.8
N.D.
-5.0
-0.1
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.8
N.D.
-4.9
-0.1
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.6
N.D.
-3.6
-0.6
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.8
N.D.
-4.5
-0.0
N.D.
auxotrophy
YNB Glucose
-0.8
N.D.
-4.4
-0.0
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-2.1
N.D.
-3.0
+0.1
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-1.7
N.D.
-3.9
+0.7
N.D.
growth on oleic acid
YNB Glucose
-0.3
N.D.
-4.4
+0.0
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-1.1
N.D.
-2.9
-0.0
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.7
N.D.
-3.5
+0.3
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
N.D.
-2.9
-0.5
N.D.
growth on ricinoleic acid
YNB Glucose
+0.3
N.D.
-3.6
+0.2
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-1.0
N.D.
-1.8
-0.3
N.D.
growth on oleic acid
YNB Oleic Acid
-0.3
N.D.
-2.4
+0.3
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.3
N.D.
-2.0
-0.1
N.D.
auxotrophy
Dropout Complete
-2.6
N.D.
+0.5
-0.3
N.D.
ypd buoyancy
40 hrs Low Buoyancy
+0.3
N.D.
-1.3
-0.2
N.D.
ypd buoyancy
40 hrs Median Buoyancy
+0.0
N.D.
-0.7
-0.1
N.D.
growth on ypd
YPD
+1.0
N.D.
-1.0
-0.4
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-1.7
N.D.
+2.3
+0.1
N.D.
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