Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14259 and 14260 are separated by 177 nucleotides14260 and 14261 are separated by 140 nucleotides14261 and 14262 are separated by 406 nucleotides14262 and 14263 are separated by 255 nucleotides mRNA_5891: 14259 - K11788 ADE5 phosphoribosylamine--glycine ligase / phosphoribosylformylglycinamidine cyclo-ligase, at 1,182,342 to 1,185,171 14259 mRNA_5892: 14260 - Hypothetical Protein, at 1,185,349 to 1,185,740 14260 mRNA_5893: 14261 - KOG1748 Acyl carrier protein/NADH-ubiquinone oxidoreductase, NDUFAB1/SDAP subunit, at 1,185,881 to 1,186,514 14261 mRNA_5894: 14262 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SUPERFAMILY--SSF57701, at 1,186,921 to 1,189,596 14262 mRNA_5895: 14263 - K17255 GDI1_2 Rab GDP dissociation inhibitor, at 1,189,852 to 1,191,772 14263
Group Condition mRNA_5891 mRNA_5892 mRNA_5893 mRNA_5894 mRNA_5895
14259 14260 14261 14262 14263
buoyancy on lipid accumulation 40 hrs Low Buoyancy -4.9 -0.1 N.D. -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -4.7 -0.4 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -5.0 -0.1 N.D. -0.1 N.D.
auxotrophy YNB Glucose -4.4 -0.0 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -4.5 -0.0 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -4.4 +0.0 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -3.6 -0.6 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose -3.6 +0.2 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -3.9 +0.7 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -2.9 -0.5 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -3.5 +0.3 N.D. -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -2.9 -0.0 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.0 +0.1 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.8 -0.3 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -2.0 -0.1 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid -2.4 +0.3 N.D. +0.1 N.D.
growth on ypd YPD -1.0 -0.4 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -1.3 -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.7 -0.1 N.D. -0.1 N.D.
auxotrophy Dropout Complete +0.5 -0.3 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +2.3 +0.1 N.D. +0.3 N.D.
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