Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14261 and 14262 are separated by 406 nucleotides14262 and 14263 are separated by 255 nucleotides14263 and 14264 are separated by 201 nucleotides14264 and 14265 are separated by 104 nucleotides mRNA_5893: 14261 - KOG1748 Acyl carrier protein/NADH-ubiquinone oxidoreductase, NDUFAB1/SDAP subunit, at 1,185,881 to 1,186,514 14261 mRNA_5894: 14262 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SUPERFAMILY--SSF57701, at 1,186,921 to 1,189,596 14262 mRNA_5895: 14263 - K17255 GDI1_2 Rab GDP dissociation inhibitor, at 1,189,852 to 1,191,772 14263 mRNA_5896: 14264 - K07573 CSL4, EXOSC1 exosome complex component CSL4, at 1,191,974 to 1,192,832 14264 mRNA_5897: 14265 - Interpro Mitochondrial import receptor subunit or translocase, at 1,192,937 to 1,193,713 14265
Group Condition mRNA_5893 mRNA_5894 mRNA_5895 mRNA_5896 mRNA_5897
14261 14262 14263 14264 14265
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 N.D. -0.6 -0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.5 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. -0.6 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.4 N.D. -0.1 -0.3
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.5 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.2 N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. -0.7 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. -0.0 -0.2
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 N.D. -0.3 -0.1
growth on ypd YPD N.D. -0.2 N.D. -0.4 +0.3
auxotrophy YNB Glucose N.D. -0.5 N.D. +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. -0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. -0.3 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. -0.6 +0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. +0.2 +0.0
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