Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14266 and 14267 are separated by 266 nucleotides14267 and 14268 are separated by 2785 nucleotides14268 and 14269 are separated by 168 nucleotides14269 and 14270 are separated by 417 nucleotides mRNA_5898: 14266 - Hypothetical Protein, at 1,194,829 to 1,196,243 14266 mRNA_5899: 14267 - K00038 E1.1.1.53 3alpha(or 20beta)-hydroxysteroid dehydrogenase, at 1,196,510 to 1,197,611 14267 mRNA_5900: 14268 - KOG4778 Mitochondrial ribosomal protein L28, at 1,200,397 to 1,201,096 14268 mRNA_5901: 14269 - K07204 RAPTOR regulatory associated protein of mTOR, at 1,201,265 to 1,206,244 14269 mRNA_5902: 14270 - K08336 ATG12 ubiquitin-like protein ATG12, at 1,206,662 to 1,207,258 14270
Group Condition mRNA_5898 mRNA_5899 mRNA_5900 mRNA_5901 mRNA_5902
14266 14267 14268 14269 14270
facs on lipid accumulation Sorted High BODIPY Gate -0.6 +0.1 N.D. N.D. -3.9
facs on lipid accumulation Sorted Low BODIPY Gate -2.9 +0.3 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.3 N.D. N.D. -2.0
ypd buoyancy 40 hrs No Buoyancy Separation -1.6 -0.4 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 N.D. N.D. -1.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 N.D. N.D. -1.5
growth on oleic acid YNB Oleic Acid -1.3 +0.1 N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.0 N.D. N.D. -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.1 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 N.D. N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.3 N.D. N.D. -0.2
growth on oleic acid YNB Glucose +0.1 -0.0 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.1 -0.0 N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 N.D. N.D. -0.1
growth on ypd YPD +0.5 +0.5 N.D. N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.9 -0.1 N.D. N.D. -0.0
auxotrophy Dropout Complete +1.2 -0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose +1.3 -0.1 N.D. N.D. +0.1
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