Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14277 and 14278 are separated by 695 nucleotides14278 and 14279 are separated by 202 nucleotides14279 and 14280 are separated by 546 nucleotides14280 and 14281 are separated by 109 nucleotides mRNA_5909: 14277 - K00077 panE, apbA 2-dehydropantoate 2-reductase, at 1,220,676 to 1,222,238 14277 mRNA_5910: 14278 - K11094 SNRPB2 U2 small nuclear ribonucleoprotein B'', at 1,222,934 to 1,224,150 14278 mRNA_5911: 14279 - ProSitePatterns-EGF-like domain signature 1.-PS00022,ProSitePatterns-EGF-like domain signature 2.-PS01186,ProSiteProfiles-EGF-like domain profile.-PS50026, at 1,224,353 to 1,227,750 14279 mRNA_5912: 14280 - K11375 ELP4 elongator complex protein 4, at 1,228,297 to 1,229,962 14280 mRNA_5913: 14281 - K14455 GOT2 aspartate aminotransferase, mitochondrial, at 1,230,072 to 1,231,795 14281
Group Condition mRNA_5909 mRNA_5910 mRNA_5911 mRNA_5912 mRNA_5913
14277 14278 14279 14280 14281
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 -2.8 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 -1.8 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -2.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.0 +0.0 -2.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.6 -1.6 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 -0.6 -1.0
growth on ypd YPD N.D. N.D. -0.2 -1.4 -0.2
auxotrophy Dropout Complete N.D. N.D. -0.3 -1.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -1.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 -1.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -0.5 -0.8
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.4 -0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.8 -0.2
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.4 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 -1.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -1.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 -0.0 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.0 -0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.1 +0.4 -0.3
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