Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14315 and 14316 are separated by 476 nucleotides14316 and 14317 are separated by 869 nucleotides14317 and 14318 are separated by 1640 nucleotides14318 and 14319 are separated by 197 nucleotides mRNA_5947: 14315 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 1,298,029 to 1,303,055 14315 mRNA_5948: 14316 - HMMPfam-Zinc finger C-x8-C-x5-C-x3-H type-PF14608,ProSiteProfiles-Zinc finger C3H1-type profile.-PS50103,SMART-zinc finger-SM00356, at 1,303,532 to 1,306,602 14316 mRNA_5949: 14317 - K01046 E3.1.1.3 triacylglycerol lipase, at 1,307,472 to 1,308,620 14317 mRNA_5950: 14318 - KOG2130 Phosphatidylserine-specific receptor PtdSerR, contains JmjC domain, at 1,310,261 to 1,312,291 14318 mRNA_5951: 14319 - HMMPfam-Peptidase of plants and bacteria-PF04450, at 1,312,489 to 1,313,415 14319
Group Condition mRNA_5947 mRNA_5948 mRNA_5949 mRNA_5950 mRNA_5951
14315 14316 14317 14318 14319
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.9 N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.0 +0.2 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.4 N.D. -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.8 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.4 N.D. -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 +0.1 N.D. -0.0 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 N.D. -0.2 +0.0
growth on ricinoleic acid YNB Glucose -0.3 +0.1 N.D. -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.0 N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.3 N.D. -0.3 +0.2
auxotrophy YNB Glucose +0.2 -0.3 N.D. -0.3 +0.1
auxotrophy Dropout Complete +0.2 -0.1 N.D. -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 -0.1 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.5 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.1 N.D. -0.0 +0.3
growth on oleic acid YNB Glucose -0.7 +0.4 N.D. +0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 N.D. +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.5 N.D. +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 +0.7 N.D. +0.4 +0.1
growth on ypd YPD +0.8 +0.7 N.D. +0.0 -0.3
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