Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14317 and 14318 are separated by 1640 nucleotides14318 and 14319 are separated by 197 nucleotides14319 and 14320 are separated by 351 nucleotides14320 and 14321 are separated by 270 nucleotides mRNA_5949: 14317 - K01046 E3.1.1.3 triacylglycerol lipase, at 1,307,472 to 1,308,620 14317 mRNA_5950: 14318 - KOG2130 Phosphatidylserine-specific receptor PtdSerR, contains JmjC domain, at 1,310,261 to 1,312,291 14318 mRNA_5951: 14319 - HMMPfam-Peptidase of plants and bacteria-PF04450, at 1,312,489 to 1,313,415 14319 mRNA_5952: 14320 - K15198 BDP1, TFC5 transcription factor TFIIIB component B'', at 1,313,767 to 1,316,568 14320 mRNA_5953: 14321 - KOG2084 Predicted histone tail methylase containing SET domain, at 1,316,839 to 1,317,817 14321
Group Condition mRNA_5949 mRNA_5950 mRNA_5951 mRNA_5952 mRNA_5953
14317 14318 14319 14320 14321
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -0.4 N.D. -0.3
auxotrophy Dropout Complete N.D. -0.5 +0.0 N.D. -0.2
growth on ypd YPD N.D. +0.0 -0.3 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 +0.0 N.D. -0.2
auxotrophy YNB Glucose N.D. -0.3 +0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.2 -0.0 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.2 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 +0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.1 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.1 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.3 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.0 N.D. +0.2
growth on oleic acid YNB Glucose N.D. +0.0 +0.4 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.3 N.D. +0.0
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