Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14321 and 14322 are separated by 124 nucleotides14322 and 14323 are separated by 986 nucleotides14323 and 14324 are separated by 661 nucleotides14324 and 14325 are separated by 836 nucleotides mRNA_5953: 14321 - KOG2084 Predicted histone tail methylase containing SET domain, at 1,316,839 to 1,317,817 14321 mRNA_5954: 14322 - HMMPfam-Domain of unknown function (DUF2427)-PF10348,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665, at 1,317,942 to 1,318,810 14322 mRNA_5955: 14323 - K11654 SMARCA5, SNF2H, ISWI SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, at 1,319,797 to 1,323,881 14323 mRNA_5956: 14324 - KOG2238 Uncharacterized conserved protein TEX2, contains PH domain, at 1,324,543 to 1,328,402 14324 mRNA_5957: 14325 - HMMPfam-Eukaryotic cytochrome b561-PF03188,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665,SUPERFAMILY--SSF49344, at 1,329,239 to 1,331,004 14325
Group Condition mRNA_5953 mRNA_5954 mRNA_5955 mRNA_5956 mRNA_5957
14321 14322 14323 14324 14325
growth on ypd YPD -0.2 -0.0 N.D. -0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.0 N.D. -0.1 -0.2
auxotrophy YNB Glucose -0.1 -0.4 N.D. +0.0 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.3 N.D. +0.0 +0.3
auxotrophy Dropout Complete -0.2 -0.0 N.D. +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.6 N.D. +0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.3 N.D. -0.1 +0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. +0.0 +0.1
growth on oleic acid YNB Glucose +0.0 +0.0 N.D. +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.1 N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.3 N.D. +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.2 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.0 -0.0 N.D. +0.3 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.3 N.D. +0.3 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.3 N.D. +0.2 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.1 N.D. +0.5 +0.4
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