Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14324 and 14325 are separated by 836 nucleotides14325 and 14326 are separated by 455 nucleotides14326 and 14327 are separated by 496 nucleotides14327 and 14328 are separated by 311 nucleotides mRNA_5956: 14324 - KOG2238 Uncharacterized conserved protein TEX2, contains PH domain, at 1,324,543 to 1,328,402 14324 mRNA_5957: 14325 - HMMPfam-Eukaryotic cytochrome b561-PF03188,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665,SUPERFAMILY--SSF49344, at 1,329,239 to 1,331,004 14325 mRNA_5958: 14326 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 1,331,460 to 1,334,052 14326 mRNA_5959: 14327 - HMMPfam-Domain of unknown function (DUF1793)-PF08760, at 1,334,549 to 1,337,487 14327 mRNA_5960: 14328 - Hypothetical Protein, at 1,337,799 to 1,338,235 14328
Group Condition mRNA_5956 mRNA_5957 mRNA_5958 mRNA_5959 mRNA_5960
14324 14325 14326 14327 14328
auxotrophy YNB Glucose +0.0 +0.0 -0.1 -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.2 -0.0 -0.2 -0.1
auxotrophy Dropout Complete +0.0 -0.0 -0.1 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 +0.1 +0.1 +0.1
growth on oleic acid YNB Glucose +0.1 +0.0 -0.1 -0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.3 +0.1 -0.2 -0.2
growth on oleic acid YNB Oleic Acid +0.0 +0.1 -0.0 +0.3 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.3 -0.0 -0.2 +0.0
growth on ypd YPD -0.1 -0.5 +0.5 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.0 -0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.6 -0.2 +0.5 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 -0.2 +0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.3 +0.3 -0.0 -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.2 +0.1 +0.2 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 +0.2 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 +0.2 +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.4 -0.2 +0.1 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.2 +0.2 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.3 +0.3 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 +0.3 -0.0 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.4 +0.2 +0.2 +0.3
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