Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14361 and 14362 are separated by 206 nucleotides14362 and 14363 are separated by 150 nucleotides14363 and 14364 are separated by 535 nucleotides14364 and 14365 are separated by 321 nucleotides mRNA_5993: 14361 - K12847 USP39, SAD1 U4/U6.U5 tri-snRNP-associated protein 2, at 1,402,184 to 1,404,821 14361 mRNA_5994: 14362 - K14774 UTP25, DEF U3 small nucleolar RNA-associated protein 25, at 1,405,028 to 1,407,712 14362 mRNA_5995: 14363 - K14539 LSG1 large subunit GTPase 1, at 1,407,863 to 1,410,284 14363 mRNA_5996: 14364 - BLAST ran GTPase activating protein 1 [Capsaspora owcza..., at 1,410,820 to 1,412,089 14364 mRNA_5997: 14365 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 1,412,411 to 1,414,490 14365
Group Condition mRNA_5993 mRNA_5994 mRNA_5995 mRNA_5996 mRNA_5997
14361 14362 14363 14364 14365
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.8 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.9 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.3 -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.0 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.4 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.4 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. N.D. -0.0 +0.2
growth on ypd YPD N.D. N.D. N.D. -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.0 +0.2
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.5 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.5 +0.2
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