Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14363 and 14364 are separated by 535 nucleotides14364 and 14365 are separated by 321 nucleotides14365 and 14366 are separated by 492 nucleotides14366 and 14367 are separated by 142 nucleotides mRNA_5995: 14363 - K14539 LSG1 large subunit GTPase 1, at 1,407,863 to 1,410,284 14363 mRNA_5996: 14364 - BLAST ran GTPase activating protein 1 [Capsaspora owcza..., at 1,410,820 to 1,412,089 14364 mRNA_5997: 14365 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 1,412,411 to 1,414,490 14365 mRNA_5998: 14366 - Hypothetical Protein, at 1,414,983 to 1,417,635 14366 mRNA_5999: 14367 - KOG1064 RAVE (regulator of V-ATPase assembly) complex subunit RAV1/DMX protein, WD repeat superfamily, at 1,417,778 to 1,422,818 14367
Group Condition mRNA_5995 mRNA_5996 mRNA_5997 mRNA_5998 mRNA_5999
14363 14364 14365 14366 14367
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.1 -0.1 -3.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 +0.4 -0.1 -1.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.9 +0.3 -0.0 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 +0.3 +0.1 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.8 -0.2 -0.4 +0.9
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 +0.0 -0.1 -0.3
auxotrophy Dropout Complete N.D. -0.3 -0.1 -0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.2 -0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 -0.0 -0.3 +0.3
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.0 +0.0 -0.2
auxotrophy YNB Glucose N.D. -0.0 +0.2 -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.5 +0.2 -0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.2 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.3 +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.2 -0.3 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 -0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 -0.0 +0.4
growth on oleic acid YNB Glucose N.D. +0.1 +0.2 +0.1 +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.1 -0.2 +1.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.5 +0.1 -0.1 +0.6
growth on ypd YPD N.D. -0.1 +0.3 +0.1 +1.0
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