Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14368 and 14369 are separated by 180 nucleotides14369 and 14370 are separated by 324 nucleotides14370 and 14371 are separated by 432 nucleotides14371 and 14372 are separated by 178 nucleotides mRNA_6000: 14368 - KOG2517 Ribulose kinase and related carbohydrate kinases, at 1,423,087 to 1,425,937 14368 mRNA_6001: 14369 - KOG1575 Voltage-gated shaker-like K+ channel, subunit beta/KCNAB, at 1,426,118 to 1,427,755 14369 mRNA_6002: 14370 - KOG4178 Soluble epoxide hydrolase, at 1,428,080 to 1,429,229 14370 mRNA_6003: 14371 - K14792 RRP5, PDCD11 rRNA biogenesis protein RRP5, at 1,429,662 to 1,434,924 14371 mRNA_6004: 14372 - SUPERFAMILY--SSF52047, at 1,435,103 to 1,436,492 14372
Group Condition mRNA_6000 mRNA_6001 mRNA_6002 mRNA_6003 mRNA_6004
14368 14369 14370 14371 14372
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.2 -0.5 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.3 -0.5 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.1 +0.1 N.D. -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.4 -0.7 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 -0.3 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.2 -1.0 N.D. -0.2
auxotrophy YNB Glucose +0.1 -0.2 -0.2 N.D. -0.3
auxotrophy Dropout Complete +0.1 +0.1 -0.1 N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 +0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 +0.3 N.D. -0.6
growth on ricinoleic acid YNB Glucose +0.0 +0.1 +0.2 N.D. -0.4
growth on ypd YPD +0.4 -0.0 -0.3 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 +0.5 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.3 -0.1 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.0 -0.4 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.2 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.0 -0.3 N.D. +0.0
growth on oleic acid YNB Glucose -0.2 +0.3 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.0 -0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.2 -0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.2 -0.0 N.D. +0.4
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