Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14394 and 14395 are separated by 780 nucleotides14395 and 14396 are separated by 26 nucleotides14396 and 14397 are separated by 203 nucleotides14397 and 14398 are separated by 252 nucleotides mRNA_6026: 14394 - HMMPfam-Zeta toxin-PF06414,SUPERFAMILY--SSF52540, at 1,476,488 to 1,478,196 14394 mRNA_6027: 14395 - HMMPfam-Cysteine-rich secretory protein family-PF00188,PRINTS-Allergen V5/Tpx-1 family signature-PR00837,SMART-SCP / Tpx-1 / Ag5 / PR-1 / Sc7 family of extracellular domains.-SM00198,SUPERFAMILY--SSF55797, at 1,478,977 to 1,480,198 14395 mRNA_6028: 14396 - KOG2521 Uncharacterized conserved protein, at 1,480,225 to 1,481,311 14396 mRNA_6029: 14397 - K01056 PTH1, pth, spoVC peptidyl-tRNA hydrolase, PTH1 family, at 1,481,515 to 1,482,449 14397 mRNA_6030: 14398 - KOG4036 Uncharacterized conserved protein, at 1,482,702 to 1,483,838 14398
Group Condition mRNA_6026 mRNA_6027 mRNA_6028 mRNA_6029 mRNA_6030
14394 14395 14396 14397 14398
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.6 -0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.0 +0.2 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.4 -0.0 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 -0.0 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 +0.1 N.D. +0.3
auxotrophy Dropout Complete N.D. -0.1 -0.0 N.D. -0.0
growth on ypd YPD N.D. +0.2 -0.1 N.D. -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.0 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.0 N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. -0.2 +0.3 N.D. +0.1
auxotrophy YNB Glucose N.D. +0.0 +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.1 +0.2 N.D. +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +1.1 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 +0.3 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 +0.4 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +1.6 +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +2.1 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +1.7 +0.0 N.D. +0.4
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