Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14395 and 14396 are separated by 26 nucleotides14396 and 14397 are separated by 203 nucleotides14397 and 14398 are separated by 252 nucleotides14398 and 14399 are separated by 334 nucleotides mRNA_6027: 14395 - HMMPfam-Cysteine-rich secretory protein family-PF00188,PRINTS-Allergen V5/Tpx-1 family signature-PR00837,SMART-SCP / Tpx-1 / Ag5 / PR-1 / Sc7 family of extracellular domains.-SM00198,SUPERFAMILY--SSF55797, at 1,478,977 to 1,480,198 14395 mRNA_6028: 14396 - KOG2521 Uncharacterized conserved protein, at 1,480,225 to 1,481,311 14396 mRNA_6029: 14397 - K01056 PTH1, pth, spoVC peptidyl-tRNA hydrolase, PTH1 family, at 1,481,515 to 1,482,449 14397 mRNA_6030: 14398 - KOG4036 Uncharacterized conserved protein, at 1,482,702 to 1,483,838 14398 mRNA_6031: 14399 - KOG2890 Predicted membrane protein, at 1,484,173 to 1,485,684 14399
Group Condition mRNA_6027 mRNA_6028 mRNA_6029 mRNA_6030 mRNA_6031
14395 14396 14397 14398 14399
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 +0.2 N.D. +0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.0 N.D. -0.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.7 +0.1 N.D. +0.3 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.6 -0.1 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid -0.2 +0.3 N.D. +0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.0 N.D. +0.1 +0.1
auxotrophy Dropout Complete -0.1 -0.0 N.D. -0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.0 N.D. +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.0 N.D. +0.3 -0.2
auxotrophy YNB Glucose +0.0 +0.1 N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 N.D. +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.0 N.D. -0.0 +0.3
growth on oleic acid YNB Glucose +0.1 +0.2 N.D. +0.4 -0.3
growth on ricinoleic acid YNB Glucose +0.2 +0.2 N.D. +0.2 -0.2
growth on ypd YPD +0.2 -0.1 N.D. -0.0 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.3 N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.6 +0.0 N.D. -0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.4 N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 +0.0 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +2.1 +0.2 N.D. -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.7 +0.0 N.D. +0.4 +0.0
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