Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14415 and 14416 are separated by 287 nucleotides14416 and 14417 are separated by 539 nucleotides14417 and 14418 are separated by 262 nucleotides14418 and 14419 are separated by 360 nucleotides mRNA_6047: 14415 - K17268 COPE coatomer subunit epsilon, at 1,515,323 to 1,516,944 14415 mRNA_6048: 14416 - Interpro WSTF, HB1, Itc1p, MBD9 motif 1, at 1,517,232 to 1,519,356 14416 mRNA_6049: 14417 - K08517 SEC22 vesicle transport protein SEC22, at 1,519,896 to 1,521,308 14417 mRNA_6050: 14418 - K03942 NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, at 1,521,571 to 1,523,726 14418 mRNA_6051: 14419 - Hypothetical Protein, at 1,524,087 to 1,525,475 14419
Group Condition mRNA_6047 mRNA_6048 mRNA_6049 mRNA_6050 mRNA_6051
14415 14416 14417 14418 14419
auxotrophy Dropout Complete -1.2 +0.3 -0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.6 +0.1 -0.3 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 +0.0 -0.0 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.8 +0.2 +0.0 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.6 +0.1 +0.1 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.5 N.D. -0.0
auxotrophy YNB Glucose -0.3 +0.2 +0.0 N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 +0.3 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.4 +1.0 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 +0.0 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.2 -0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.1 +0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.0 -0.0 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.4 +0.5 +0.1 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.0 +0.8 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 +0.4 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.0 +0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.3 +0.0 N.D. -0.2
growth on ypd YPD -0.4 +0.5 +0.2 N.D. +0.3
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