Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14427 and 14428 are separated by 474 nucleotides14428 and 14429 are separated by 309 nucleotides14429 and 14430 are separated by 525 nucleotides14430 and 14431 are separated by 285 nucleotides mRNA_6059: 14427 - SUPERFAMILY--SSF52047, at 1,543,443 to 1,544,992 14427 mRNA_6060: 14428 - K14806 DDX31, DBP7 ATP-dependent RNA helicase DDX31/DBP7, at 1,545,467 to 1,548,419 14428 mRNA_6061: 14429 - KOG3772 M-phase inducer phosphatase, at 1,548,729 to 1,549,422 14429 mRNA_6062: 14430 - K11121 SIR2 NAD-dependent histone deacetylase SIR2, at 1,549,948 to 1,551,724 14430 mRNA_6063: 14431 - KOG0519 Sensory transduction histidine kinase, at 1,552,010 to 1,556,333 14431
Group Condition mRNA_6059 mRNA_6060 mRNA_6061 mRNA_6062 mRNA_6063
14427 14428 14429 14430 14431
growth on ypd YPD +0.2 N.D. -0.3 -0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. -0.1 -0.2 -0.1
auxotrophy YNB Glucose +0.2 N.D. -0.2 -0.2 -0.0
auxotrophy Dropout Complete -0.0 N.D. -0.2 -0.1 +0.1
growth on oleic acid YNB Glucose +0.0 N.D. +0.1 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.3 -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.3 +0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.3 +0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.2 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.1 +0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.0 +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.4 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.7 -0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.5 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.2 +0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. +0.7 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.4 +0.4 +0.0
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