Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14433 and 14434 are separated by 395 nucleotides14434 and 14435 are separated by 98 nucleotides14435 and 14436 are separated by 249 nucleotides14436 and 14437 are separated by 220 nucleotides mRNA_6065: 14433 - KOG1082 Histone H3 (Lys9) methyltransferase SUV39H1/Clr4, required for transcriptional silencing, at 1,560,376 to 1,561,281 14433 mRNA_6066: 14434 - K20363 YIP1, YIPF5 protein transport protein YIP1, at 1,561,677 to 1,562,701 14434 mRNA_6067: 14435 - K14798 LTV1 protein LTV1, at 1,562,800 to 1,564,980 14435 mRNA_6068: 14436 - K01855 PUS3, DEG1 tRNA pseudouridine38/39 synthase, at 1,565,230 to 1,567,371 14436 mRNA_6069: 14437 - KOG0198 MEKK and related serine/threonine protein kinases, at 1,567,592 to 1,572,715 14437
Group Condition mRNA_6065 mRNA_6066 mRNA_6067 mRNA_6068 mRNA_6069
14433 14434 14435 14436 14437
auxotrophy YNB Glucose -0.4 N.D. N.D. -0.8 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. N.D. -0.4 -0.2
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. -0.6 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. N.D. -0.1 +0.1
auxotrophy Dropout Complete -0.4 N.D. N.D. +0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.4 -0.0
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. N.D. +0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. -0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.4 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. -0.3 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.1 -0.0
growth on ypd YPD -0.1 N.D. N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. +0.1 +0.4
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. +0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. +0.0 +0.2
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