Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14436 and 14437 are separated by 220 nucleotides14437 and 14438 are separated by 163 nucleotides14438 and 14439 are separated by 1302 nucleotides14439 and 14440 are separated by 214 nucleotides mRNA_6068: 14436 - K01855 PUS3, DEG1 tRNA pseudouridine38/39 synthase, at 1,565,230 to 1,567,371 14436 mRNA_6069: 14437 - KOG0198 MEKK and related serine/threonine protein kinases, at 1,567,592 to 1,572,715 14437 mRNA_6070: 14438 - K14552 NAN1, UTP17, WDR75 NET1-associated nuclear protein 1 (U3 small nucleolar RNA-associated protein 17), at 1,572,879 to 1,575,986 14438 mRNA_6071: 14439 - Hypothetical Protein, at 1,577,289 to 1,577,537 14439 mRNA_6072: 14440 - Hypothetical Protein, at 1,577,752 to 1,580,946 14440
Group Condition mRNA_6068 mRNA_6069 mRNA_6070 mRNA_6071 mRNA_6072
14436 14437 14438 14439 14440
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.1 N.D. N.D. -0.8
auxotrophy YNB Glucose -0.8 -0.0 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.6 -0.0 N.D. N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.1 N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.2 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.0 N.D. N.D. -0.1
growth on ypd YPD +0.3 -0.2 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.0 N.D. N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.0 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.4 N.D. N.D. -0.1
growth on oleic acid YNB Glucose -0.2 +0.0 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.2 N.D. N.D. -0.2
auxotrophy Dropout Complete +0.0 +0.0 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.1 N.D. N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.4 +0.2 N.D. N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.4 N.D. N.D. +0.1
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