Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14445 and 14446 are separated by 238 nucleotides14446 and 14447 are separated by 113 nucleotides14447 and 14448 are separated by 342 nucleotides14448 and 14449 are separated by 86 nucleotides mRNA_6077: 14445 - KOG3869 Uncharacterized conserved protein, at 1,590,853 to 1,592,368 14445 mRNA_6078: 14446 - HMMPfam-Essential protein Yae1, N terminal-PF09811, at 1,592,607 to 1,593,994 14446 mRNA_6079: 14447 - K12776 MEK1 meiosis-specific serine/threonine-protein kinase MEK1, at 1,594,108 to 1,596,398 14447 mRNA_6080: 14448 - KOG4379 Uncharacterized conserved protein (tumor antigen CML66 in humans), at 1,596,741 to 1,598,901 14448 mRNA_6081: 14449 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 1,598,988 to 1,602,551 14449
Group Condition mRNA_6077 mRNA_6078 mRNA_6079 mRNA_6080 mRNA_6081
14445 14446 14447 14448 14449
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.4 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -0.1 N.D. +0.4
auxotrophy Dropout Complete N.D. -0.1 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.1 N.D. -0.1
growth on ypd YPD N.D. +0.3 -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.2 N.D. +0.2
auxotrophy YNB Glucose N.D. +0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.1 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.2 -0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -0.1 N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.5 -0.2 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.0 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.5 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 -0.0 N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.6 -0.2 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 -0.0 N.D. +0.2
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