Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14448 and 14449 are separated by 86 nucleotides14449 and 14450 are separated by 764 nucleotides14450 and 14451 are separated by 194 nucleotides14451 and 14452 are separated by 164 nucleotides mRNA_6080: 14448 - KOG4379 Uncharacterized conserved protein (tumor antigen CML66 in humans), at 1,596,741 to 1,598,901 14448 mRNA_6081: 14449 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 1,598,988 to 1,602,551 14449 mRNA_6082: 14450 - K03010 RPB2, POLR2B DNA-directed RNA polymerase II subunit RPB2, at 1,603,316 to 1,607,774 14450 mRNA_6083: 14451 - Hypothetical Protein, at 1,607,969 to 1,610,179 14451 mRNA_6084: 14452 - SUPERFAMILY--SSF54427, at 1,610,344 to 1,611,077 14452
Group Condition mRNA_6080 mRNA_6081 mRNA_6082 mRNA_6083 mRNA_6084
14448 14449 14450 14451 14452
growth on ypd YPD N.D. -0.0 N.D. -0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 N.D. -0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.2 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. +0.3 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 N.D. +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -0.0 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 N.D. +0.3 +0.0
auxotrophy YNB Glucose N.D. +0.0 N.D. +0.4 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. +0.1 +0.3
growth on oleic acid YNB Glucose N.D. +0.2 N.D. +0.5 -0.0
auxotrophy Dropout Complete N.D. +0.0 N.D. +0.4 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.3 +0.3
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