Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14460 and 14461 are separated by 250 nucleotides14461 and 14462 are separated by 175 nucleotides14462 and 14463 are separated by 793 nucleotides14463 and 14464 are separated by 918 nucleotides mRNA_6092: 14460 - K06639 CDC14 cell division cycle 14, at 1,628,198 to 1,631,375 14460 mRNA_6093: 14461 - HMMPfam-Permease for cytosine/purines, uracil, thiamine, allantoin-PF02133,PIRSF--PIRSF002744, at 1,631,626 to 1,633,865 14461 mRNA_6094: 14462 - K16738 NDE1, NUDE nuclear distribution protein NudE, at 1,634,041 to 1,636,155 14462 mRNA_6095: 14463 - KOG0054 Multidrug resistance-associated protein/mitoxantrone resistance protein, ABC superfamily, at 1,636,949 to 1,643,604 14463 mRNA_6096: 14464 - K05721 FGD2 FYVE, RhoGEF and PH domain containing 2, at 1,644,523 to 1,649,566 14464
Group Condition mRNA_6092 mRNA_6093 mRNA_6094 mRNA_6095 mRNA_6096
14460 14461 14462 14463 14464
growth on ypd YPD -0.1 -0.1 -0.2 +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 +0.1 -0.4 +0.0
auxotrophy Dropout Complete +0.2 -0.2 -0.3 +0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.1 -0.2 -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.3 -0.1 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 +0.0 -0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.2 -0.4 -0.0 +0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 -0.2 +0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -0.0 +0.2 +0.1
growth on oleic acid YNB Glucose +0.3 -0.1 +0.1 +0.2 +0.0
auxotrophy YNB Glucose +0.3 +0.1 -0.0 +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 +0.1 -0.0 +0.3
growth on ricinoleic acid YNB Glucose +0.4 +0.1 -0.2 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.1 +0.1 +0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 +0.0 -0.1 +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.5 +0.2 +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.4 +0.5 +0.1 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 +0.4 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.2 +0.4 +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.3 -0.0 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.2 +0.5 +0.3 +0.2
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