Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14461 and 14462 are separated by 175 nucleotides14462 and 14463 are separated by 793 nucleotides14463 and 14464 are separated by 918 nucleotides14464 and 14465 are separated by 288 nucleotides mRNA_6093: 14461 - HMMPfam-Permease for cytosine/purines, uracil, thiamine, allantoin-PF02133,PIRSF--PIRSF002744, at 1,631,626 to 1,633,865 14461 mRNA_6094: 14462 - K16738 NDE1, NUDE nuclear distribution protein NudE, at 1,634,041 to 1,636,155 14462 mRNA_6095: 14463 - KOG0054 Multidrug resistance-associated protein/mitoxantrone resistance protein, ABC superfamily, at 1,636,949 to 1,643,604 14463 mRNA_6096: 14464 - K05721 FGD2 FYVE, RhoGEF and PH domain containing 2, at 1,644,523 to 1,649,566 14464 mRNA_6097: 14465 - KOG4313 Thiamine pyrophosphokinase, at 1,649,855 to 1,650,926 14465
Group Condition mRNA_6093 mRNA_6094 mRNA_6095 mRNA_6096 mRNA_6097
14461 14462 14463 14464 14465
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 -0.4 +0.0 -0.1
growth on ypd YPD -0.1 -0.2 +0.1 +0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.2 -0.3 +0.2 +0.2
growth on oleic acid YNB Oleic Acid -0.4 -0.0 +0.2 +0.2 +0.2
auxotrophy Dropout Complete -0.2 -0.3 +0.3 -0.0 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.1 +0.1 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.2 +0.2 +0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.0 +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 +0.2 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 -0.0 +0.3 +0.0
auxotrophy YNB Glucose +0.1 -0.0 +0.2 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.1 +0.5 -0.0 +0.1
growth on oleic acid YNB Glucose -0.1 +0.1 +0.2 +0.0 +0.3
growth on ricinoleic acid YNB Glucose +0.1 -0.2 +0.2 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.4 +0.4 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.4 +0.3 +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.2 +0.3 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.1 +0.1 -0.0 +0.8
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.0 +0.3 +0.4 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.5 +0.3 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.5 +0.1 +0.5 -0.0
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