Fitness for 3 genes in Rhodosporidium toruloides IFO0880

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500 nt 14474 and 14475 are separated by 331 nucleotides14475 and 14476 are separated by 226 nucleotides mRNA_6106: 14474 - K08332 VAC8 vacuolar protein 8, at 223 to 3,658 14474 mRNA_6107: 14475 - K14401 CPSF1, CFT1 cleavage and polyadenylation specificity factor subunit 1, at 3,990 to 10,005 14475 mRNA_6108: 14476 - Hypothetical Protein, at 10,232 to 11,111 14476
Group Condition mRNA_6106 mRNA_6107 mRNA_6108
14474 14475 14476
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.9
facs on lipid accumulation Sorted No BODIPY Gate -1.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. -0.3
growth on oleic acid YNB Glucose -0.6 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. -1.0
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2
auxotrophy YNB Glucose -0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.6
growth on ypd YPD -0.1 N.D. +0.1
auxotrophy Dropout Complete -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.4
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