Fitness for 4 genes in Rhodosporidium toruloides IFO0880

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500 nt 14474 and 14475 are separated by 331 nucleotides14475 and 14476 are separated by 226 nucleotides14476 and 14477 are separated by 1086 nucleotides mRNA_6106: 14474 - K08332 VAC8 vacuolar protein 8, at 223 to 3,658 14474 mRNA_6107: 14475 - K14401 CPSF1, CFT1 cleavage and polyadenylation specificity factor subunit 1, at 3,990 to 10,005 14475 mRNA_6108: 14476 - Hypothetical Protein, at 10,232 to 11,111 14476 mRNA_6109: 14477 - KOG1477 SPRY domain-containing proteins, at 12,198 to 14,626 14477
Group Condition mRNA_6106 mRNA_6107 mRNA_6108 mRNA_6109
14474 14475 14476 14477
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.9 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. -0.8 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.1 N.D. -0.2 +0.1
growth on oleic acid YNB Glucose -0.6 N.D. -0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. -0.3 +0.4
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.4 -0.2
auxotrophy YNB Glucose -0.2 N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. -1.0 +0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.3 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.3 +0.2
growth on ypd YPD -0.1 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.6 +0.3
auxotrophy Dropout Complete -0.0 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.0 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.4 +0.3
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