Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14474 and 14475 are separated by 331 nucleotides14475 and 14476 are separated by 226 nucleotides14476 and 14477 are separated by 1086 nucleotides14477 and 14478 are separated by 440 nucleotides mRNA_6106: 14474 - K08332 VAC8 vacuolar protein 8, at 223 to 3,658 14474 mRNA_6107: 14475 - K14401 CPSF1, CFT1 cleavage and polyadenylation specificity factor subunit 1, at 3,990 to 10,005 14475 mRNA_6108: 14476 - Hypothetical Protein, at 10,232 to 11,111 14476 mRNA_6109: 14477 - KOG1477 SPRY domain-containing proteins, at 12,198 to 14,626 14477 mRNA_6110: 14478 - Hypothetical Protein, at 15,067 to 15,769 14478
Group Condition mRNA_6106 mRNA_6107 mRNA_6108 mRNA_6109 mRNA_6110
14474 14475 14476 14477 14478
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.9 -0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -1.1 N.D. -0.2 +0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. -0.8 +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.1 -0.2 -0.2
auxotrophy YNB Glucose -0.2 N.D. -0.0 -0.2 -0.3
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.4 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. +0.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.3 +0.2 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.3 +0.5 -0.4
growth on oleic acid YNB Glucose -0.6 N.D. -0.1 +0.0 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. -0.3 +0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.6 +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.2 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. -1.0 +0.1 +0.5
auxotrophy Dropout Complete -0.0 N.D. +0.3 +0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 +0.2 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.1 +0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.4 +0.3 -0.5
growth on ypd YPD -0.1 N.D. +0.1 -0.0 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.0 +0.6 -0.1
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