Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14484 and 14485 are separated by 603 nucleotides14485 and 14486 are separated by 449 nucleotides14486 and 14487 are separated by 176 nucleotides14487 and 14488 are separated by 149 nucleotides mRNA_6116: 14484 - Hypothetical Protein, at 27,856 to 28,790 14484 mRNA_6117: 14485 - BLAST expressed protein [Schizophyllum commune H4-8], at 29,394 to 30,967 14485 mRNA_6118: 14486 - K03164 TOP2 DNA topoisomerase II, at 31,417 to 38,158 14486 mRNA_6119: 14487 - K15280 SLC35C2 solute carrier family 35, member C2, at 38,335 to 41,391 14487 mRNA_6120: 14488 - K05954 FNTB protein farnesyltransferase subunit beta, at 41,541 to 43,349 14488
Group Condition mRNA_6116 mRNA_6117 mRNA_6118 mRNA_6119 mRNA_6120
14484 14485 14486 14487 14488
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. -1.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.3 N.D. -0.0 N.D.
growth on ypd YPD +0.1 -0.4 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.2 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.3 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose +0.1 +0.0 N.D. -0.0 N.D.
auxotrophy Dropout Complete +0.5 -0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.0 N.D. +0.1 N.D.
auxotrophy YNB Glucose +0.4 -0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.3 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.6 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.3 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.6 N.D. +0.1 N.D.
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