Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14485 and 14486 are separated by 449 nucleotides14486 and 14487 are separated by 176 nucleotides14487 and 14488 are separated by 149 nucleotides14488 and 14489 are separated by 6297 nucleotides mRNA_6117: 14485 - BLAST expressed protein [Schizophyllum commune H4-8], at 29,394 to 30,967 14485 mRNA_6118: 14486 - K03164 TOP2 DNA topoisomerase II, at 31,417 to 38,158 14486 mRNA_6119: 14487 - K15280 SLC35C2 solute carrier family 35, member C2, at 38,335 to 41,391 14487 mRNA_6120: 14488 - K05954 FNTB protein farnesyltransferase subunit beta, at 41,541 to 43,349 14488 mRNA_6121: 14489 - KOG3607 Meltrins, fertilins and related Zn-dependent metalloproteinases of the ADAMs family, at 49,647 to 51,616 14489
Group Condition mRNA_6117 mRNA_6118 mRNA_6119 mRNA_6120 mRNA_6121
14485 14486 14487 14488 14489
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.1 N.D. -1.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -1.2 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. +0.1 N.D. -0.7
growth on ypd YPD -0.4 N.D. +0.1 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.2 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.1 N.D. -0.3
auxotrophy Dropout Complete -0.2 N.D. -0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.1 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. +0.0 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.5 N.D. +0.5
growth on oleic acid YNB Glucose +0.0 N.D. -0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.3 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.4 N.D. -0.6
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.0 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.1 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.5 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.1 N.D. +0.5
auxotrophy YNB Glucose -0.0 N.D. -0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. +0.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. +0.1 N.D. +0.2
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