Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14486 and 14487 are separated by 176 nucleotides14487 and 14488 are separated by 149 nucleotides14488 and 14489 are separated by 6297 nucleotides14489 and 14490 are separated by 2521 nucleotides mRNA_6118: 14486 - K03164 TOP2 DNA topoisomerase II, at 31,417 to 38,158 14486 mRNA_6119: 14487 - K15280 SLC35C2 solute carrier family 35, member C2, at 38,335 to 41,391 14487 mRNA_6120: 14488 - K05954 FNTB protein farnesyltransferase subunit beta, at 41,541 to 43,349 14488 mRNA_6121: 14489 - KOG3607 Meltrins, fertilins and related Zn-dependent metalloproteinases of the ADAMs family, at 49,647 to 51,616 14489 mRNA_6122: 14490 - SUPERFAMILY--SSF53098, at 54,138 to 56,327 14490
Group Condition mRNA_6118 mRNA_6119 mRNA_6120 mRNA_6121 mRNA_6122
14486 14487 14488 14489 14490
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. -1.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.2 N.D. -0.7 +0.8
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -0.7 -0.3
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.0 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 N.D. +0.5 -0.8
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. -0.3 -0.2
growth on ypd YPD N.D. +0.1 N.D. -0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.5 -0.7
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. +0.5 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 N.D. -0.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 N.D. -0.5 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.3 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. +0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +0.4 -0.2
auxotrophy YNB Glucose N.D. -0.1 N.D. +0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.5 N.D. -0.2 +0.3
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