Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14487 and 14488 are separated by 149 nucleotides14488 and 14489 are separated by 6297 nucleotides14489 and 14490 are separated by 2521 nucleotides14490 and 14491 are separated by 464 nucleotides mRNA_6119: 14487 - K15280 SLC35C2 solute carrier family 35, member C2, at 38,335 to 41,391 14487 mRNA_6120: 14488 - K05954 FNTB protein farnesyltransferase subunit beta, at 41,541 to 43,349 14488 mRNA_6121: 14489 - KOG3607 Meltrins, fertilins and related Zn-dependent metalloproteinases of the ADAMs family, at 49,647 to 51,616 14489 mRNA_6122: 14490 - SUPERFAMILY--SSF53098, at 54,138 to 56,327 14490 mRNA_6123: 14491 - BLAST DNA polymerase [Rhizoctonia solani], at 56,792 to 57,276 14491
Group Condition mRNA_6119 mRNA_6120 mRNA_6121 mRNA_6122 mRNA_6123
14487 14488 14489 14490 14491
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -1.3 -0.3 -0.6
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.4 -0.4 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. +0.5 -0.8 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.2 N.D. -0.7 +0.8 -0.6
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.3 -0.2 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.5 -0.7 -1.2
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.4 -0.2 -1.5
auxotrophy Dropout Complete -0.1 N.D. +0.1 -0.2 -1.1
auxotrophy YNB Glucose -0.1 N.D. +0.4 -0.1 -1.1
growth on oleic acid YNB Glucose -0.0 N.D. -0.0 -0.7 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.2 +0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +0.5 -0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.1 +0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.7 -0.3 +1.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 +0.4 +0.1
growth on ypd YPD +0.1 N.D. -0.4 -0.0 +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. -0.6 +0.2 +0.7
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. -0.2 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.3 +0.3 +1.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.2 +0.2 +1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.5 +0.5 +1.5
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