Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14489 and 14490 are separated by 2521 nucleotides14490 and 14491 are separated by 464 nucleotides14491 and 14492 are separated by 171 nucleotides14492 and 14493 are separated by 3414 nucleotides mRNA_6121: 14489 - KOG3607 Meltrins, fertilins and related Zn-dependent metalloproteinases of the ADAMs family, at 49,647 to 51,616 14489 mRNA_6122: 14490 - SUPERFAMILY--SSF53098, at 54,138 to 56,327 14490 mRNA_6123: 14491 - BLAST DNA polymerase [Rhizoctonia solani], at 56,792 to 57,276 14491 mRNA_6124: 14492 - Hypothetical Protein, at 57,448 to 58,239 14492 mRNA_6125: 14493 - KOG1477 SPRY domain-containing proteins, at 61,654 to 62,774 14493
Group Condition mRNA_6121 mRNA_6122 mRNA_6123 mRNA_6124 mRNA_6125
14489 14490 14491 14492 14493
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 -0.3 -0.6 -0.2 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.8 -0.6 -0.1 -2.3
growth on ricinoleic acid YNB Glucose +0.4 -0.4 -1.9 -0.1 -0.3
growth on oleic acid YNB Oleic Acid -0.3 -0.2 -1.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.2 -1.5 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.7 -1.2 +0.2 -0.3
growth on oleic acid YNB Glucose -0.0 -0.7 +0.1 -0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.8 -1.1 -0.0 +0.4
auxotrophy YNB Glucose +0.4 -0.1 -1.1 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.3 +1.2 -0.2 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.3 -0.3 +0.1 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 -0.5 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 +0.2 +0.7 -0.2 -0.4
auxotrophy Dropout Complete +0.1 -0.2 -1.1 +0.4 +0.6
growth on ypd YPD -0.4 -0.0 +1.0 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.3 -0.2 +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.4 +0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.5 +1.5 -0.5 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.3 +0.2 +0.0 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.3 +1.4 -0.2 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.2 +1.0 -0.0 +0.4
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