Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14495 and 14496 are separated by 215 nucleotides14496 and 14497 are separated by 173 nucleotides14497 and 14498 are separated by 91 nucleotides14498 and 14499 are separated by 511 nucleotides mRNA_6127: 14495 - KOG0772 Uncharacterized conserved protein, contains WD40 repeat, at 68,190 to 70,680 14495 mRNA_6128: 14496 - BLAST DEHA2B11858p [Debaryomyces hansenii CBS767], at 70,896 to 71,507 14496 mRNA_6129: 14497 - Hypothetical Protein, at 71,681 to 73,838 14497 mRNA_6130: 14498 - K11786 STH1_SNF2 ATP-dependent helicase STH1/SNF2, at 73,930 to 78,529 14498 mRNA_6131: 14499 - K01057 PGLS, pgl, devB 6-phosphogluconolactonase, at 79,041 to 80,063 14499
Group Condition mRNA_6127 mRNA_6128 mRNA_6129 mRNA_6130 mRNA_6131
14495 14496 14497 14498 14499
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -1.2 -0.1 N.D.
auxotrophy YNB Glucose N.D. -0.6 -0.4 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 -0.2 -0.8 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 -0.8 -0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -0.6 -0.8 N.D.
auxotrophy Dropout Complete N.D. -0.5 -0.6 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 -0.2 -0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.5 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 +0.0 -0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.2 -0.1 N.D.
growth on oleic acid YNB Glucose N.D. -0.4 -0.3 -0.1 N.D.
growth on ypd YPD N.D. -0.1 -0.6 -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.1 -0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.6 -0.3 -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 -0.4 +0.3 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.3 +0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +0.5 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 +0.2 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 +0.2 +0.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +0.7 +1.2 N.D.
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