Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14499 and 14500 are separated by 316 nucleotides14500 and 14501 are separated by 923 nucleotides14501 and 14502 are separated by 941 nucleotides14502 and 14503 are separated by 490 nucleotides mRNA_6131: 14499 - K01057 PGLS, pgl, devB 6-phosphogluconolactonase, at 79,041 to 80,063 14499 mRNA_6132: 14500 - K20630 CHN1_2, ARHGAP2_3 chimaerin, at 80,380 to 84,392 14500 mRNA_6133: 14501 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 85,316 to 87,118 14501 mRNA_6134: 14502 - KOG1477 SPRY domain-containing proteins, at 88,060 to 89,781 14502 mRNA_6135: 14503 - K08614 ADAM28 disintegrin and metalloproteinase domain-containing protein 28, at 90,272 to 93,233 14503
Group Condition mRNA_6131 mRNA_6132 mRNA_6133 mRNA_6134 mRNA_6135
14499 14500 14501 14502 14503
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.2 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 -0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.1 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.0 N.D. +0.0
growth on oleic acid YNB Glucose N.D. -0.3 +0.1 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 -0.1 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.2 N.D. +0.1
growth on ypd YPD N.D. -0.3 +0.3 N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.3 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.3 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 +0.1 N.D. +0.4
auxotrophy YNB Glucose N.D. +0.2 +0.0 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +0.0 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.1 N.D. -0.2
auxotrophy Dropout Complete N.D. +0.2 +0.0 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.4 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 -0.2 N.D. +0.7
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