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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14499 and 14500 are separated by 316 nucleotides
14500 and 14501 are separated by 923 nucleotides
14501 and 14502 are separated by 941 nucleotides
14502 and 14503 are separated by 490 nucleotides
mRNA_6131: 14499 - K01057 PGLS, pgl, devB 6-phosphogluconolactonase, at 79,041 to 80,063
14499
mRNA_6132: 14500 - K20630 CHN1_2, ARHGAP2_3 chimaerin, at 80,380 to 84,392
14500
mRNA_6133: 14501 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 85,316 to 87,118
14501
mRNA_6134: 14502 - KOG1477 SPRY domain-containing proteins, at 88,060 to 89,781
14502
mRNA_6135: 14503 - K08614 ADAM28 disintegrin and metalloproteinase domain-containing protein 28, at 90,272 to 93,233
14503
Group
Condition
mRNA
_6131
mRNA
_6132
mRNA
_6133
mRNA
_6134
mRNA
_6135
14499
14500
14501
14502
14503
auxotrophy
YNB Glucose 75mM Arginine
N.D.
-0.3
-0.2
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
-0.2
-0.2
N.D.
-0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.5
-0.2
N.D.
+0.2
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.3
-0.1
N.D.
+0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-0.3
-0.0
N.D.
+0.0
growth on oleic acid
YNB Glucose
N.D.
-0.3
+0.1
N.D.
-0.0
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-0.4
-0.2
N.D.
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.0
-0.1
N.D.
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-0.4
-0.1
N.D.
+0.3
ypd buoyancy
40 hrs High Buoyancy
N.D.
+0.0
-0.2
N.D.
+0.1
growth on ypd
YPD
N.D.
-0.3
+0.3
N.D.
+0.0
growth on ricinoleic acid
YNB Glucose
N.D.
-0.2
+0.3
N.D.
-0.0
growth on oleic acid
YNB Oleic Acid
N.D.
-0.3
+0.3
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
-0.5
+0.1
N.D.
+0.4
auxotrophy
YNB Glucose
N.D.
+0.2
+0.0
N.D.
-0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
-0.1
+0.1
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
+0.2
+0.0
N.D.
+0.0
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.3
+0.1
N.D.
-0.2
auxotrophy
Dropout Complete
N.D.
+0.2
+0.0
N.D.
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.1
+0.4
N.D.
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
+0.4
-0.2
N.D.
+0.7
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