Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14502 and 14503 are separated by 490 nucleotides14503 and 14504 are separated by 215 nucleotides14504 and 14505 are separated by 793 nucleotides14505 and 14506 are separated by 365 nucleotides mRNA_6134: 14502 - KOG1477 SPRY domain-containing proteins, at 88,060 to 89,781 14502 mRNA_6135: 14503 - K08614 ADAM28 disintegrin and metalloproteinase domain-containing protein 28, at 90,272 to 93,233 14503 mRNA_6136: 14504 - Hypothetical Protein, at 93,449 to 95,989 14504 mRNA_6137: 14505 - KOG2227 Pre-initiation complex, subunit CDC6, AAA+ superfamily ATPase, at 96,783 to 98,967 14505 mRNA_6138: 14506 - Hypothetical Protein, at 99,333 to 99,963 14506
Group Condition mRNA_6134 mRNA_6135 mRNA_6136 mRNA_6137 mRNA_6138
14502 14503 14504 14505 14506
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -0.2 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.1 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.1 N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. -0.0 +0.1 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 N.D. +0.2
growth on oleic acid YNB Glucose N.D. -0.0 -0.3 N.D. +0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.4 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.4 N.D. +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.0 N.D. +0.2
auxotrophy YNB Glucose N.D. -0.1 +0.0 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.1 N.D. +0.3
growth on ypd YPD N.D. +0.0 -0.1 N.D. +0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 +0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 -0.1 N.D. +0.7
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.1 N.D. +0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +0.0 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.2 N.D. +1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 -0.4 N.D. +0.9
auxotrophy Dropout Complete N.D. +0.1 +0.0 N.D. +0.9
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 +0.1 N.D. +0.4
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