Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14513 and 14514 are separated by 482 nucleotides14514 and 14515 are separated by 1769 nucleotides14515 and 14516 are separated by 661 nucleotides14516 and 14517 are separated by 426 nucleotides mRNA_6145: 14513 - HMMPfam-Protein of unknown function (DUF2456)-PF10445, at 115,513 to 116,915 14513 mRNA_6146: 14514 - K02925 RP-L3e, RPL3 large subunit ribosomal protein L3e, at 117,398 to 118,882 14514 mRNA_6147: 14515 - Hypothetical Protein, at 120,652 to 120,840 14515 mRNA_6148: 14516 - Hypothetical Protein, at 121,502 to 122,312 14516 mRNA_6149: 14517 - Hypothetical Protein, at 122,739 to 123,475 14517
Group Condition mRNA_6145 mRNA_6146 mRNA_6147 mRNA_6148 mRNA_6149
14513 14514 14515 14516 14517
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -0.3 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. N.D. +0.5 -2.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.5 -0.6
growth on oleic acid YNB Glucose +0.0 N.D. N.D. -0.5 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. -0.3 +0.1
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. -0.7 +0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.6 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. +0.2 -0.7
auxotrophy Dropout Complete -0.2 N.D. N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. N.D. -0.3 +0.4
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. N.D. -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. -0.6 +0.5
growth on ypd YPD +0.1 N.D. N.D. +0.5 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. +0.6 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.2 -0.0
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