Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14527 and 14528 are separated by 259 nucleotides14528 and 14529 are separated by 58 nucleotides14529 and 14530 are separated by 192 nucleotides14530 and 14531 are separated by 304 nucleotides mRNA_6159: 14527 - Hypothetical Protein, at 143,889 to 144,383 14527 mRNA_6160: 14528 - Hypothetical Protein, at 144,643 to 145,145 14528 mRNA_6161: 14529 - Hypothetical Protein, at 145,204 to 145,497 14529 mRNA_6162: 14530 - K11427 DOT1L, DOT1 histone-lysine N-methyltransferase, H3 lysine-79 specific, at 145,690 to 148,355 14530 mRNA_6163: 14531 - HMMPfam-Autophagy protein 16 (ATG16)-PF08614, at 148,660 to 149,493 14531
Group Condition mRNA_6159 mRNA_6160 mRNA_6161 mRNA_6162 mRNA_6163
14527 14528 14529 14530 14531
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 -0.5 -3.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.8 +0.7 -4.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.5 +0.3 -2.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.2 -1.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.5 -0.3 -0.5
growth on ypd YPD N.D. N.D. -0.9 +0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.3 +0.2 -0.7
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.7 +0.2 -1.5
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.5 -0.3 +0.2
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 +0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 +0.5 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 -0.1 +0.5
growth on oleic acid YNB Glucose N.D. N.D. -0.5 -0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 +0.1 +0.3
auxotrophy YNB Glucose N.D. N.D. +0.4 -0.3 +0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.0 +0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 -0.1 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 +0.8 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.4 +0.8
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