Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14529 and 14530 are separated by 192 nucleotides14530 and 14531 are separated by 304 nucleotides14531 and 14532 are separated by 126 nucleotides14532 and 14533 are separated by 191 nucleotides mRNA_6161: 14529 - Hypothetical Protein, at 145,204 to 145,497 14529 mRNA_6162: 14530 - K11427 DOT1L, DOT1 histone-lysine N-methyltransferase, H3 lysine-79 specific, at 145,690 to 148,355 14530 mRNA_6163: 14531 - HMMPfam-Autophagy protein 16 (ATG16)-PF08614, at 148,660 to 149,493 14531 mRNA_6164: 14532 - BLAST ECSIT domain-containing protein [Rhizoctonia solani A..., at 149,620 to 151,505 14532 mRNA_6165: 14533 - HMMPfam-WD domain, G-beta repeat-PF00400,HMMPfam-F-box-like-PF12937,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF50978,SUPERFAMILY--SSF81383, at 151,697 to 155,852 14533
Group Condition mRNA_6161 mRNA_6162 mRNA_6163 mRNA_6164 mRNA_6165
14529 14530 14531 14532 14533
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.5 -3.7 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 +0.7 -4.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.3 -2.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 -1.9 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.3 -0.5 N.D. +0.2
growth on ypd YPD -0.9 +0.0 +0.0 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.2 -0.7 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.5 -0.3 +0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.2 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.2 -1.5 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.5 -0.6 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.3 -0.3 N.D. +0.1
auxotrophy Dropout Complete -0.2 -0.4 +0.1 N.D. +0.2
growth on oleic acid YNB Glucose -0.5 -0.1 +0.6 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.1 +0.5 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.3 N.D. +0.1
auxotrophy YNB Glucose +0.4 -0.3 +0.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.8 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.0 +0.3 +0.3 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 +0.6 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.4 +0.8 N.D. +0.0
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